What Differentiates Humic and Fulvic Acids?

By Richard Lamar, PhD
Director of Humic Research
Bio Huma Netics, Inc.

For centuries, humic acids (HA) were thought to be composed of much larger molecules than those found in fulvic acids (FA). However, the application of Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS), which separates molecules on the basis of molecular weight, demonstrates that the molecular weights of the two fractions both fall in the range of 200–800 Daltons (Da), with most of the molecules having molecular weights in the range of 200–400 Da (Figure 1). To give context, carbon (C) weighs 12 Da, oxygen (O) weighs 16 Da, and hydrogen (H) weighs 1 Da. Thus, phenol molecules (an aromatic organic compound, also called carbolic acid), which have 6 C, 1 O, and 11 H atoms, weigh 99 Da. [Read more…]

JoVE Video Journal Publication: Quantification of Humic and Fulvic Acids

Dr. Richard T. Lamar and Dr. Hiarhi Monda of our Humic Research Laboratory, with assistance from analytical chemist Ryan Fountain, have published a methodology video in the biochemistry section of the peer-reviewed online video journal, JoVE.

The video, Quantification of Humic and Fulvic Acids in Humate Ores, DOC, Humified Materials and Humic Substance-Containing Commercial Products, shows the step-by-step laboratory methodology (the New Standard Method) for gravimetric quantification of humic substances (e.g., humic and fulvic acids) on an ash-free basis, in dry and liquid materials from soft coals (i.e., oxidized and non-oxidized lignite and sub-bituminous coal), humate ores and shales, peats, composts and commercial fertilizers and soil amendments.

In the video introduction, Dr. Lamar states, “The New Standard Method for quantification of humic acids provides a more accurate and precise analysis compared to the existing regulatorily accepted methods, and it also provides a standard method for pure hydrophobic fulvic acid quantification. The advantage of this protocol is that it provides a gravimetric analysis of humic and hydrophobic fulvic acid concentrations on an ash-free basis, and the extraction process has been optimized to obtain the highest recoveries of both humic and fulvic acids from samples.

At the video’s conclusion, Dr. Monda states, “Following this procedure, the dry humic and fulvic acids obtained can be used for characterization purposes, such as the carbon-13 and the proton NMR electron resonance, and the ultrahigh resolution mass spectrometry, among other useful techniques. This can be used for characterization of the humus chemistry, as well as being a useful tool to dig deep into the structure-activity relationship with plant fitness and the underlying plant defense mechanisms.

Direct link to video on the JoVE Website: https://www.jove.com/v/61233/quantification-humic-fulvic-acids-humate-ores-doc-humified-materials (A free subscription will be required to view the entire video on the JoVE Website.)

From the JoVE Website: Filmed at the world’s top scientific institutions, JoVE videos bring to life the intricate details of cutting-edge experiments enabling efficient learning and replication of new research methods and technologies. JoVE is a peer-reviewed scientific video journal that is indexed in PubMed and Web of Science.

The Huma Gro Farmer Podcast: Episode 9—Growing Premium Corn

Today on Episode 9 we’re talking about Growing Premium Corn with Huma Gro® Products. Our guests include Mr. Lyndon Smith, President and CEO of Bio Huma Netics, Inc.—the maker of Huma Gro®, Fertilgold® Organics, Mesa Verde Humates, and several other product lines—and Mr. Jason Garcia, Florida Sales Manager and Agronomist for Bio Huma Netics, Inc. Growing corn is quite a complicated topic, so in this podcast we walk through the typical growth cycle of corn and discuss the nutrients that are needed at each stage. We also discuss products that you might need because of common deficiencies or pests and products that you might want to add in order to achieve premium quality for your crop. We review the needs of both grain corn and sweet corn and discuss nutritional needs differences between the two.

Products Discussed

Micro Carbon Technology®, 44 Mag®, Activol®, Breakout®, Calcium, Cobalt, Copper, Crop-Gard®, D-Fend®, Fertil Humus®, Fertil Soil®, Fertilgold® XT, Fulvi Pro®, Golden Pro®, Huma Burst® 1–3 mm, Huma Burst® K Hume®, Huma Pro®, Jackpot®, Lucky 7®, Max Pak®, Molybdenum, Promax®, Proud 3®, Pur Cal®, Sulfur, Start-L™, Super Nitro®, Super Phos®, Super Potassium®, Vitol®, X-Tend®, Z-Max®, Zap®

Other Resources

Book mentioned during the podcast: For the Love of Soil, by Nicole Masters.

Humic Acids vs. Compost

Two of the most common methods used for rapidly increasing soil organic matter and improving soil biology are to add compost or to add humic substances. There are pros and cons with each. [Read more…]

Our Humic Advantage

Humic substances, among the most wondrous products of nature, are the recycled essential residues of life. Plants harvest the sun’s energy and create life from that energy plus the carbon and oxygen they pull out of the air and the minerals they pull out of the soil. When plants die, all of their components are decomposed through the aid of microorganisms and mineralization, then returned to the soil as organic matter. [Read more…]

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